Extracellular fluid flow and chloride content modulate H+ transport by osteoclasts

Depth resolved granular transport driven by shearing fluid flow

We investigate granular transport by a fluid flow under steady-state driving conditions, from the bed-load regime to the suspension regime, with an experimental system based on a conical rheometer. The mean granular volume fraction φg , the mean granular velocity ug , and the fluid velocity uf are obtained as a function of depth inside the bed using refractive index matching and particle-tracki...

Sodium Chloride Transport by Rabbit

The results of the present study indicate that NaC1 transport by in vitro rabbit gallbladder must be a consequence of a neutral coupled carriermediated mechanism that ultimately results in the active absorption of both ions; pure electrical coupling between the movements of Na and C1 can be excluded on the grounds of electrophysiologic considerations. Studies on the unidirectional influxes of N...

Cytoplasmic pH regulation and chloride/bicarbonate exchange in avian osteoclasts.

Osteoclasts resorb bone by first attaching to the bone surface and then secreting protons into an isolated extracellular compartment formed at the cell-bone attachment site. This secretion of protons (local acidification) is required to solubilize bone hydroxyapatite crystals and for activity of bone collagen-degrading acid proteases. However, the large quantity of protons required, 2 mol/mol o...

Chloride transport by high-resistance heterocellular epithelia.

Sodium-coupled chloride transport by epithelial tissues.

There is compelling evidence that active Cl absorption by a variety of epithelia, widely distributed throughout the animal kingdom, is the result of an electrically neutral Na-coupled transport process at the luminal membrane and that the energy for transcellular Cl movement is derived from the Na gradient across that barrier. These co-transport processes are found predominantly in "leaky" or "...